Status of MDR and Plasmid Profiling of ESBL producing E. coli and Klebsiella spp. isolated from milk of Bovine in Gangetic Plain Zone of Uttar Pradesh

Nowadays resistance to antibiotics of β-lactam groups is expanding rapidly and one the latest challenges faced by scientific community due therapeutic failure. In present study total 180 milk samples were collected from two district Gangetic Plain Zone Uttar Pradesh (India). Total 65 (36.11%) isolates confirmed comprising 44 (24.4%) E. coli 21 (11.67%) Klebsiella spp. PCR analysis using uidA gene 16S rRNA respectively, 35/180 (19.44%) as ESBL producers, constituting 12 (6.67%) 23 (12.8%) All positive subjected antimicrobial pattern 17 10 different classes. this all found 100% resistant cefotaxime, cefpodoxime, ceftriazone ampicillin. Both revealed susceptibility non-β-lactam except aminoglycosides class for which both sensitive. The multi-drug was assessed MAR index. Out 80.0% MDR with 78.0% 83.0% Overall observed ranging 5 09 14 antibiotics. Plasmid profiling 71.42% carrying plasmids average molecular weight ranged between 1.0 Kb > number1-4.